TABLE 1.
Bacterial strains and plasmids used
| Strain or plasmid | Genotype, phenotype, or properties | Source or reference |
|---|---|---|
| E. coli strains | ||
| JM109 | e14− (McrA−) recA1 endA1 gyrA96 thi-1 hsdR17(rK− mK+) supE44 relA1 Δ(lac-proAB) [F′ traD36+proAB+lacIqZΔM15] | Stratagene |
| DH5α | F− φ80dlacΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK− mK+) supE44 λ−thi-1 gyrA96 relA | Invitrogen |
| P. aeruginosa strains | ||
| PAO1 | IATS serotype O5; P. aeruginosa type strain (ATCC 33351) | 9a |
| KHP-wapR | PAO1 derivative; Gmr-marked wapR mutant | This study |
| KHP-migA | PAO1 derivative; Gmr-marked migA mutant | This study |
| KHP-migA/rmd | PAO1 derivative; Gmr-marked migA rmd double mutant | This study |
| RML-ΔC | PAO1 derivative; unmarked rmlC mutant | 20 |
| Plasmids | ||
| pEX18Ap | Counterselectable, mobilizable suicide vector for gene replacement; contains OriT and sacB; Apr | 11 |
| pPS856 | Gmr-selectable Flp recombinase target (FRT) cassette vector for generation of marked or unmarked mutants via gene interruption | 11 |
| pFLP2 | bhr FLP recombinanse-producing plasmid; contains sacB; Apr | 11 |
| pFV327-TGm | pEX18Ap containing rmd interrupted with a Gmr cassette | 23 |
| pUCP26 | pUC18-derived broad-host-range cloning vector | 30a |
| pKPJL01 | wapR in pUCP26 (KpnI/PstI) | This study |
| pKPJL02 | migA in pUCP26 (EcoRI/BamHI) | This study |