FIG. 2.

Rescue of YPH499-HIS-GAL-ALG7 by complementation with either the human ALG7 or Mv1751 gene. The conditional lethal mutant YPH499-HIS-GAL-ALG7 was transformed with the pRS426Met plasmids carrying either the human ALG7 (HsALG7) or the archaeal Mv1751 (MvALG7). The transformed cells were then streaked onto plates containing minimal medium lacking histidine and containing either galactose (II) or glucose (III) and incubated at 30°C. Panel III clearly shows that both the human and archaeal alg7 genes rescue the conditional lethal phenotype of YPH499-HIS-GAL and complement the yeast alg7.