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Indirect immunofluorescence microscopy comparing the distributions of the ER marker BiP and the expressed Mv1751 in S. cerevisiae. FLAG-tagged Mv1751 is clearly visible inside cells containing the MvALG7 plasmid, while cells containing the empty pRS426Met plasmid showed no fluorescence. Costaining with BiP showed nearly complete overlapping with Mv1751. Cells were stained with DAPI (4′,6′-diamidino-2-phenylindole) to detect chromosomal DNA. PC, phase contrast.
