FIG. 1.

Analysis of the expression of known pathogenesis-related genes containing the CpxR consensus sequence. (A) Sequence alignment of the regulatory regions of icm/dot genes (icmR, icmV, and icmW), the icm/dot accessory gene lvgA, and two IDTS-encoding genes (sidM and sidH). The putative consensus sequence is indicated by boldface type, and the distances to the first ATG codon are also indicated. The complementary strand is shown for genes in which the consensus regulatory element was identified on the reverse strand (icmV, icmW, and lvgA). (B) Expression of lacZ fusions in wild-type L. pneumophila and the cpxR deletion mutant. The expression of lacZ fusions of three icm/dot genes (icmR, icmV, and icmW), the lvgA gene, two IDTS-encoding genes (sidM and sidH) that contain the CpxR consensus regulatory element, and lepB that does not contain the consensus regulatory element in wild-type strain JR32 (shaded bars) and the cpxR deletion mutant OG2002 (open bars) was examined. β-Galactosidase activity was determined as described in Materials and Methods. The data (in Miller units [MU]) are the averages ± standard deviations (error bars) of at least three different experiments.