FIG. 8.
Icm/Dot-dependent translocation of CpxR-regulated genes. (A) Wild-type strain JR32 (grey bars) and icmT mutant GS3011 (open bars) harboring the CyaA fusion proteins (indicated below the bars) were used to infect HL-60-derived human macrophages, and the cAMP levels of the infected cells were determined as described in Materials and Methods. (B) Effect of the cpxR deletion mutant on the translocation of sidM and cegC4 cyaA fusions in wild-type strain JR32 (grey bars), cpxR mutant OG2002 (black bars), and icmT mutant GS3011 (open bars). The fusions were cloned under control of Ptac or under control of their natural promoter. The data are the means for the amount of cAMP per well obtained in three independent experiments, and the error bars indicate standard deviations.