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Subcellular localization of the C-terminally tagged pUL52 variant. RPE cells were transfected with the EGFP-expressing BAC pHG-52HA-3 encoding pUL52 with an N-terminally fused HA tag (A) or with pHG-52HA-1, which encodes the C-terminally tagged UL52 ORF (B). Three days posttransfection, cells were labeled with an anti-HA antibody (α-HA) and analyzed by confocal laser scanning microscopy.
