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. 2007 Dec 12;82(5):2065–2078. doi: 10.1128/JVI.01967-07

FIG. 9.

FIG. 9.

Expression kinetics of the UL52 protein. (A) HFF were infected with RV-HG-52HA-3 at an MOI of 3. Cells were harvested at the time points indicated, and proteins were analyzed by immunoblotting using an antibody directed against the HA tag or against the viral early protein pUL44. ni, noninfected HFF. (B) To analyze specifically viral protein expression of the immediate-early (IE), early (E), or late (L) phase of the HCMV infection cycle, HFF were infected as described for panel A and either incubated in the presence of cycloheximide for 3 h followed by incubation in medium containing actinomycin D for another 5 h (IE), incubated for 72 h in the presence of phosphonoacetic acid (E), or left without inhibitors and harvested at 72 h p.i. (L). Cell lysates were analyzed by immunoblotting with antibodies directed against the HA tag, the immediate-early protein IE1, or the early protein pUL44.