FIG. 4.

Transcription of viral mRNA (A) and replication of viral genomic RNA (B) in NA cells infected with the chimeric viruses SN-BM, SN-BG, and SN-BMBG and the parental virus SB relative to those in cells infected with SN (the quantity of SN RNA was taken as 100%). Cells were infected at an MOI of 5, and total RNA was isolated at the indicated time points. A fragment of RV N mRNA or the N gene of genomic RV RNA was reverse transcribed and subjected to quantitative PCR analysis. The data were normalized using 18S rRNA as an internal control and analyzed using the comparative threshold cycle method. Data are means (± standard deviations) of values for triplicate samples in one of two independent experiments with superimposable results. gRNA, genomic RNA.