Skip to main content
. 2007 Dec 19;82(5):2330–2338. doi: 10.1128/JVI.02327-07

FIG. 5.

FIG. 5.

Cell surface expression of RV G (A) and Western blot analysis of RV M (B) in NA cells either uninfected or infected with the chimeric viruses SN-BM, SN-BG, and SN-BMBG or with the parental viruses SN and SB at an MOI of 5 for 48 h. (A) Cells were incubated with rabbit anti-RV G polyclonal antibody followed by Alexa 488-conjugated anti-rabbit IgG. Surface expression was assessed by flow cytometry, and results were analyzed using FlowJo software. (B) Cells were subjected to Western blot analysis as described in Materials and Methods. RV M was detected using polyclonal rabbit antibody against RV M followed by Alexa Fluor 555-anti-rabbit IgG. Actin, which was detected with a polyclonal rabbit antibody against mouse actin, served as a loading control. MW indicates molecular mass markers. Proteins were visualized using a molecular imager.