TABLE 4.
Effect of single residue synonymous changes in WSN PB2 mutants on the packaging of individual vRNAs into progeny virions
| Virus | PB2 | PB1 | PA | HA | NP | NA | M | NS |
|---|---|---|---|---|---|---|---|---|
| WSN | 100.0 ± 4.0 | 100.0 ± 1.4 | 100.0 ± 2.2 | 100.0 ± 2.4 | 100.0 ± 3.6 | 100.0 ± 3.7 | 100.0 ± 1.3 | 100.0 ± 2.0 |
| PB2m731 | 102.0 ± 2.3 | 115.3 ± 8.4 | 130.9 ± 3.4 | 100.0 ± 1.5 | 88.1 ± 2.4 | 177.8 ± 4.9 | 100.7 ± 3.5 | 83.9 ± 3.8 |
| PB2m737 | 129.2 ± 2.2 | 116.8 ± 3.6 | 171.9 ± 2.7 | 100.0 ± 1.6 | 64.7 ± 2.3 | 115.5 ± 3.3 | 73.6 ± 1.0 | 99.6 ± 1.5 |
| PB2m744b | 18.9 ± 2.7 | 115.5 ± 4.5 | 41.5 ± 1.4 | 100.0 ± 4.8 | 59.4 ± 1.3 | 89.8 ± 3.6 | 110.8 ± 4.5 | 154.8 ± 5.9 |
| PB2m745 | 11.6 ± 2.2 | 16.4 ± 2.9 | 51.1 ± 4.4 | 100.0 ± 3.4 | 14.9 ± 1.2 | 57.7 ± 2.9 | 37.8 ± 0.6 | 54.7 ± 3.1 |
| PB2m748 | 85.0 ± 1.2 | 82.3 ± 4.0 | 80.4 ± 0.2 | 100.0 ± 1.4 | 63.0 ± 0.8 | 87.7 ± 1.2 | 48.0 ± 0.5 | 104.9 ± 0.9 |
| PB2m751 | 56.3 ± 0.5 | 70.6 ± 1.3 | 56.6 ± 1.3 | 100.0 ± 1.1 | 40.6 ± 0.4 | 86.6 ± 0.9 | 32.4 ± 0.5 | 61.4 ± 0.8 |
| PB2m757 | 8.2 ± 0.2 | 143.0 ± 5.2 | 81.0 ± 2.2 | 100.0 ± 1.2 | 53.8 ± 1.3 | 74.4 ± 0.9 | 90.5 ± 0.7 | 92.0 ± 2.7 |
Values given are percentages of packaging of vRNA in comparison to those of parental WSN virus ± standard deviation. Results are from two independent experiments, with assay performed in triplicate (n = 6).