TABLE 9.
Effect of synonymous changes within the highly conserved region of PA on the packaging of individual vRNAs into progeny PR/8 virions
| Virus | PB2 | PB1 | PA | HA | NP | NA | M | NS |
|---|---|---|---|---|---|---|---|---|
| PR/8 | 100.0 ± 3.0 | 100.0 ± 3.7 | 100.0 ± 2.6 | 100.0 ± 4.7 | 100.0 ± 5.4 | 100.0 ± 6.6 | 100.0 ± 2.7 | 100.0 ± 5.3 |
| PR/8 PA31a | 91.6 ± 6.9 | 74.1 ± 9.2 | 73.2 ± 2.8 | 92.5 ± 7.1 | 88.7 ± 9.5 | 96.0 ± 4.1 | 100.0 ± 4.5 | 86.6 ± 4.1 |
| PR/8 PA31b | 81.4 ± 4.5 | 64.9 ± 1.4 | 78.1 ± 3.7 | 87.2 ± 5.9 | 87.6 ± 7.3 | 93.1 ± 1.8 | 100.0 ± 4.8 | 89.4 ± 1.3 |
| PR/8 PA51 | 70.3 ± 1.9 | 67.2 ± 2.9 | 43.7 ± 3.4 | 84.4 ± 4.0 | 60.2 ± 3.8 | 80.8 ± 9.3 | 100.0 ± 1.9 | 94.2 ± 6.3 |
| PR/8 PA52 | 61.1 ± 5.6 | 53.9 ± 5.1 | 62.9 ± 2.4 | 84.3 ± 1.9 | 74.7 ± 7.2 | 85.5 ± 8.9 | 100.0 ± 8.2 | 97.8 ± 5.4 |
| PR/8 PA53 | 76.7 ± 1.9 | 43.8 ± 2.1 | 5.9 ± 0.7 | 66.2 ± 3.8 | 15.8 ± 1.1 | 73.6 ± 1.3 | 100.0 ± 6.2 | 61.4 ± 5.7 |
| PR/8 PA54 | 58.9 ± 4.8 | 48.3 ± 3.0 | 68.9 ± 2.0 | 81.2 ± 5.3 | 75.4 ± 3.0 | 77.6 ± 3.2 | 100.0 ± 5.7 | 86.4 ± 6.4 |
Values given are percentages of packaging of vRNA in comparison to those of parental PR/8 virus ± standard deviation. Results are from two independent experiments, with assay performed in triplicate (n = 6).