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. 2007 Dec 19;82(5):2286–2294. doi: 10.1128/JVI.01761-07

FIG. 4.

FIG. 4.

Virus-derived EGFP mRNA harvested at various times postinfection directs translation in vitro with similar efficiencies. (A) Phosphorimage of immunoprecipitated EGFP synthesized in rabbit reticulocytes programmed with total RNA harvested from HeLa cells infected with recombinant rwt-EGFP or M51R-M-EGFP virus at 4, 8, or 12 h postinfection (hpi). Translation reaction mixes also contained total RNA harvested from HeLa cells so that each reaction mix contained the same amount of total RNA. (B) Phosphorimage of Northern blot of EGFP mRNA samples like those used for in vitro translation reactions. (C) In vitro translation efficiencies of EGFP mRNAs relative to the efficiency of translation of EGFP mRNA harvested from HeLa cells infected with rwt-EGFP virus at 4 h postinfection.