FIG. 1.

A genetic screen identified the BVDV E1*E2p7* sequence as a transdominant inhibitor of BVDV replication. (A) Illustration of the library expression cassette within the pBabeHAZSrfcI retroviral vector. A simian virus 40 promoter drives expression of the BVDV cDNA library fragment fused to an HA tag at the amino terminus and a zeocin-selectable marker at the carboxy terminus. (B) Sequence alignment of the E1*E2p7* library insertion with the BVDV genome. The brackets descending from the pictured BVDV genome highlight the BVDV sequence present in the resistant clones. Amino acid positions relative to the BVDV NADL polyprotein are indicated.