FIG. 4.

E2 expression in MDBK-E1*E2p7* cells. (A) Putative membrane topology of BVDV E1*E2p7* (adapted from reference 41). The E2 glycoprotein is an ER-resident protein with a carboxy-terminal transmembrane anchor (right-hand black line) and a short carboxy-terminal tail (gray line). Arrows indicate sites of cleavage by host signal peptidase. (B) Lysates from MDBK-E1*E2p7*- and BVDV NADL-infected MDBK cells were assayed by Western blotting using an antibody against BVDV E2 (MAb 214). Lysates from mock-infected MDBK cells and pBabe cells were used as negative controls. Arrows indicate the expected identity of the protein in each band based on molecular mass.