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. 2007 Dec 19;82(5):2427–2436. doi: 10.1128/JVI.02158-07

FIG. 5.

FIG. 5.

The E2 ectodomain is sufficient for BVDV inhibition. (A) Organization of the E2 protein and E2 expression constructs in the pLenti6 lentiviral vector. The predicted ectodomain (black bar), transmembrane anchor (white bar), and carboxy-terminal domain (gray bar) are indicated. The numbers on the top bar show the amino acid number within E2 corresponding to the start of a particular domain (21). The light gray boxes (E1*E2p7*) correspond to the E1* and p7* sequences of E1*E2p7*. The textured gray boxes (Sig-E2 and Sig-E2*) correspond to the HA signal sequence inserted upstream of the first amino acid of the E2 protein. (B) MDBK cells were transduced with packaged lentiviral vectors expressing EGFP, E1*E2p7*, Sig-E2, or Sig-E2*. After blasticidin selection, each cell population was infected with NADLJiv90− luc. At 48 h p.i., cells from triplicate wells were harvested for luciferase assays. The graph shows the average of two independent experiments done in triplicate; error bars show the standard errors of the means.