FIG. 6.

MDBK-E1*E2p7* cells have a defect at the level of BVDV entry. (A) MDBK-E1*E2p7* and pBabe cells were infected with NADLJiv90⁻ luc in the presence of a BVDV polymerase inhibitor (4). At each time point, cells were harvested for luciferase assays. Each bar represents the mean value of duplicate wells; error bars show the standard errors of the means. The dashed line indicates the background level of the assay from naïve MDBK cells. (B) MDBK-E1*E2p7* and pBabe cells were electroporated with in vitro-transcribed BVDV NADLJiv90⁻ luc RNA. At each time point, supernatants from electroporated cells were harvested and titers were determined on MDBK cells. Each point represents the mean titer of duplicate wells; error bars show the standard errors of the means.