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. 2007 Dec 19;82(5):2250–2264. doi: 10.1128/JVI.02155-07

FIG. 6.

FIG. 6.

Assembly and secretion of VLPs in the presence of different levels of HBsAg. Intracellular (A) and extracellular (B) Huh-7 cells in 10-cm-diameter dishes (6 × 106 cells per dish) were cotransfected with 10 μg (each dish) of expression plasmids of HDAg-L (genotype 1: 16L, 25L, and 83L) and 10 μg of pHBVenv plasmids expressing different HBsAg levels. Low and high expression levels of HBsAg were from pHBVenv1 and -3, respectively. Intracellular (C) and extracellular (D) Huh-7 cells in 10-cm-diameter dishes were cotransfected with 10 μg (each dish) of expression plasmids of HDAg-L (25-L, HDAg-L expressed by a genotype 1 HDV) and serial dilutions of 10 μg of HBV genotype B (pHBVenv3) and C (pHBVenv4) plasmids. Both cells and spent media were collected on day 3 posttransfection. Analyses of intracellular and extracellular HDAg-L and equal loading of protein sample in each lane were performed as described in the legend of Fig. 2. HBsAg in spent media was quantified by using a General Biological Surdine125 B kit.