Skip to main content
. 2007 Dec 19;82(5):2250–2264. doi: 10.1128/JVI.02155-07

FIG. 7.

FIG. 7.

Assembly and secretion of HDV of different genotypes with wild-type and chimeric HBsAg-expression plasmids. (A) Schematic diagram of wild-type and chimeric S-HBsAg expressed by pHBVenv4 (▪) and pHBVenv6 (□) of genotype C. A segment of the sequence from aa 37 to 185 encompassing the CYL-I and AGL known to be critical for HDV assembly was swapped between pHBVenv4 and pHBVenv6. The other segment of the sequence from aa 37 to 226 encompassing the CYL-I, AGL, and CYL-II known to be critical for HDV assembly was also swapped between pHBVenv4 and pHBVenv6. (B) Assembly and secretion of genotype 1 HDV by wild-type and chimeric HBsAgs. (C) Assembly and secretion of genotype 2 HDV by wild-type and chimeric HBsAgs. Both cells and spent media were collected on day 9 posttransfection. Analyses similar to those described in the legend of Fig. 2 of intracellular (data not shown) and extracellular HDV RNA, HDAg, and S-HBsAg were performed.