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Assembly and secretion of HDV of different genotypes with help from the wild type (pHBVenv6) and from L61S and L62P mutations in the CYL-I region of HBsAg-expression plasmids. Both cells and spent media were collected on day 9 posttransfection. (A) Analysis of HDV RNA and HDAg in cellular lysates. (B) Analysis of HDV RNA, HDAg, HBsAg, and HBV DNA in culture media. Analyses of intracellular and extracellular HDV RNA, HDAg, and S-HBsAg were performed as described in the legend of Fig. 2.
