FIG. 9.

Assembly and secretion of genotype 2 HDV with help from the wild type (pHBVenv6), double mutations (L61S and L62P), and single mutations (L61S, L62P, I110L, T126I, I131T, S140T, and K160R) in the CYL-I and AGL regions of HBsAg expression plasmids. Both cells and spent media were collected on day 9 posttransfection. (A) Analysis of HDV RNA and HDAg in cellular lysates. (B) Analysis of HDV RNA, HDAg, and S-HBsAg in culture media. Analyses of intracellular and extracellular HDV RNA, HDAg, and S-HBsAg were performed as described in the legend of Fig. 2.