FIG. 2.

Visualization of dsRNA in Huh-7 cells constitutively replicating subgenomic JFH-1 replicon RNA. (A) 2/1 JFH-1 replicon-containing cells (panels i and iii to ix) and Huh-7 control cells (panel ii) were probed for the presence of dsRNA using monoclonal antibody J2 and counterstained, where appropriate, for NS5A (panels vi to ix). Cellular regions boxed in panels iii and v are shown enlarged in panels iv and vii to ix, respectively. The arrow indicates the colocalization of dsRNA-containing foci and NS5A protein in 2/1 replicon-containing cells. (B) 2/1 cells were treated with RNase A under either low-salt (LS) or high-salt (HS) conditions and probed for dsRNA and NS5A. Bars, 10 μm.