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Distribution of dsRNA with NS5A, core protein, ADRP, and calnexin in HCV-infected cells. Huh-7 cells infected with JFH-1 virus for 48 h were analyzed for the presence of dsRNA together with ADRP and core protein (A), NS5A and calnexin (B), or NS5A and core protein (C) by indirect immunofluorescence. The selected cellular regions indicated in panels iv are shown enlarged in panels v to viii. Arrows indicate regions of close apposition of antigens. Bars, 10 μm.
