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. 2007 Dec 19;82(5):2182–2195. doi: 10.1128/JVI.01565-07

FIG. 7.

FIG. 7.

3D reconstruction of deconvolved z-stack images generated from HCV-infected cells. Selected regions from deconvolved z-stack sections of images of cells infected with JFH-1 virus for 48 h were reconstructed as 3D images. (A) Arrowheads highlight dsRNA-containing foci juxtaposed to LDs. (B) Large and small arrowheads indicate dsRNA-containing foci located on the surface of, and those surrounded by, the calnexin-labeled ER, respectively. (C) Arrowheads indicate close connections between ADRP-coated LDs and the calnexin-stained ER. (D) Large and small arrowheads indicate foci of dsRNA situated on the surface of, and those partially bounded by, ER-associated NS5A, respectively. (E and F) Arrowheads in panel E highlight close relationships among the calnexin-labeled ER, ADRP-coated LDs, and dsRNA foci. Numbered arrowheads in panel F correspond to the range of distributions observed in virus-infected cells labeled for calnexin, dsRNA, and ADRP, as follows: arrowhead 1, a single dsRNA focus on the ER surface; arrowhead 2, a single ER-attached LD; arrowhead 3, a complex composed of a single dsRNA focus juxtaposed to an LD, situated adjacent to the ER; and arrowhead 4, an ER-associated complex composed of a single LD and multiple foci of dsRNA. Bars, 1 μm.