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Cleavage at ectopic sites containing substitutions in pac1. DNAs extracted from extracellular virions produced by the indicated viruses were digested with XhoI alone or double digested with XhoI/NcoI or XhoI/HpaI, separated by agarose electrophoresis, transferred to a nylon membrane, and hybridized with sequences cloned in plasmid pMA34 (Fig. 1). The positions of molecular mass markers are indicated to the left. Arrows identify ectopic junction (JE) and ectopic terminal (TEX) fragments.
