FIG. 3.

Electron micrographs showing cytoplasmic contents in autophagic vacuoles. IHH were infected with HCV genotype 1a (clone H77) and incubated for 5 days. Infected cells were subjected to electron microscopy. IHH showing cytoplasmic contents in autophagosome are indicated by arrows (panel a). Uninfected cells were treated similarly to the negative control cells and did not exhibit any major changes (not shown). Immunogold label showing localization for E1 protein inside and on the vacuolar membrane is indicated by arrows (panel b).