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. 2007 Dec 12;82(5):2208–2217. doi: 10.1128/JVI.01718-07

FIG. 5.

FIG. 5.

The 100-bp repeat region is sufficient to induce type I IFN. (A) Schematic of the MHV-68 genome and locations of the repeat regions along with cloning sites. (B) Induction of ISRE luciferase by the 100-bp repeat region (BglII/StyI fragment), the 40-bp repeat region (AvrI/NheI fragment), and the excised terminal repeats (NotI fragment). The levels of ISRE luciferase relative to Renilla luciferase were quantitated. Only the 100-bp repeat region induced ISRE. (C) Induction of the ISRE reporter upon transfection with the 100-bp repeat region in a plasmid and after excision from the carrier plasmid, using the sites indicated in panel A. The levels of ISRE luciferase relative to Renilla luciferase were quantitated. A plasmid with the 40-bp repeat region was included as a negative control. (D) Induction of the ISRE reporter upon transfection with a PCR product containing the last 481 nt of the 100-bp repeat region. The levels of ISRE luciferase relative to Renilla luciferase were quantitated.