FIG. 4.

Expression and immunoprecipitation of recombinant VP2 proteins. Radiolabeled VP2 proteins were expressed from pCI vectors using rabbit reticulocyte lysate (A and C) or after transfection of HEK 293T cells (B and D). The lysates were subjected to immunoprecipitation using 6E8 (A and B) or YO-60 (C and D). Proteins were analyzed after SDS-PAGE and fluorography. The images were made from a 12-h exposure of the gels to film. Molecular mass markers (in kilodaltons) are shown on the left of the gels, and the location of recombinant VP2 is indicated with an arrowhead. The pCI vector with no insert (vec) was used as a control.