FIG. 5.

Wa VP2 truncation mutagenesis and immunoprecipitations. (A) Schematic diagram of Wa VP2 truncation mutants. The engineered deletions within Wa VP2 are illustrated with amino acid numbers listed above the full-length protein. Regions of Wa VP2 predicted to be required for 6E8 or YO-60 binding are indicated. (B) Expression of mutant proteins. The Wa VP2 truncation mutants were expressed using rabbit reticulocyte lysate in the presence of [³⁵S]methionine. Proteins were analyzed after SDS-PAGE and fluorography. The images were made from a 12-h exposure of the gel to film. Molecular mass markers (in kilodaltons) are shown on the left of the gels. (C and D) Immunoprecipitations. Truncated Wa VP2 proteins were immunoprecipitated using either 6E8 (C) or YO-60 (D). Proteins were analyzed as described for panel B.