FIG. 8.

Endoglycosidase H digest of S mutants. Lysates of transfected BHK-21 cells were precipitated with concanavalin A-agarose. The proteins pulled down were exposed to endoglycosidase H treatment and then separated by SDS-polyacrylamide gel electrophoresis and transferred to a nitrocellulose membrane. S proteins were visualized with anti-Beaudette serum. Only a small portion of the S0 protein is endoglycosidase H sensitive (black arrows), whereas the majority of the glycans are endoglycosidase H resistant (open arrows).