Skip to main content
. 2008 Jan 16;82(6):2741–2751. doi: 10.1128/JVI.01712-07

FIG. 3.

FIG. 3.

Apoptosis detected by cellular DNA analysis using flow cytometry. (A) Representative DNA histograms showing the proportions of apoptotic hypodiploid nuclei detected by flow cytometry. The proportion of apoptotic cells among the cells transfected with pcDNA4-NS1 increased in a time-dependent manner, but those among untransfected cells and cells transfected with empty vector did not. The percentage of apoptotic cells detected at each time point is shown. CC, untransfected control cells; vector, cells transfected with pcDNA4 empty vector; H5N1-NS1, cells transfected with pcDNA4-NS1. (B) NCI-H292 cells treated (+) or not treated (−) with the apoptosis-inducing agent staurosporine (0.5 μM) for 24 h were included as a positive control for the experiment. (C) Proportions of apoptotic cells among the untreated control cells, cells transfected with empty vector, and cells transfected with pcDNA4-NS1. Results are expressed as means ± SD of results from three independent experiments.