FIG. 6.

Inefficient trafficking of pUL37x1 NH₂-terminal deletion mutants to both MAM and mitochondria subcellular fractions. PSS-1₂₀ cells were lipofected with empty vector (p796) or vector expressing (A) pUL37x1 Δ2-23-myc (p856) or (B) pUL37x1 Δ23-34-myc (p857) and fractionated into microsomes, MAM, mitochondria, and cytosol. A total of 10 μg of p856 transfected microsomes (2.1% of fraction), MAM (3.3%), mitochondria (8.7%), and cytosol (1.5%) and 10 μg of p857 transfected microsomes (2% of fraction), MAM (4%), mitochondria (7.9%), and cytosol (1.2%) were analyzed by Western blot analysis by using a mouse antibody to the C-terminal Myc tag (1:100, MAb9E10; BabCo). Membranes were stripped and reprobed with antibodies to organelle-specific markers as described in Fig. 2.