FIG. 3.

MIRs of mutant viruses propagated in either Vero (A) or C6/36 (B) cells. The MIR is the average of IFA-positive midguts over n (n = 21) from two replicates at 9 dpi. The standard error of the mean is represented by the error bars. The shaded horizontal bar is the estimated MID₅₀. (C) Comparison of MIRs at a standardized infectious blood meal titer of 7.5 log₁₀ PFU/ml for viruses derived in either Vero or C6/36 cells. The MIR is the average of IFA-positive midguts over n (n = 21) from two replicates at 9 dpi with standard error bars. Symbols: ▪, MRE16; •, TE/5′2J; ▵, TE/Mut-5; ⋄, TE/Mut-6; ○, TE/Mut-5+6. ‡, TE/Mut-5 does not replicate to 7.5 log₁₀ PFU/ml. *, statistically significantly different from TE/5′2J.