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. 2008 Jan 9;82(6):2792–2801. doi: 10.1128/JVI.01856-07

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FIG. 6. — Effect of SF2/ASF and ORF57 on in vitro splicing of human β-globin pre-mRNA. (A) Human β-globin pre-mRNA was incubated in NE along with increasing amounts (2, 8, and 15 pmol) of recombinant SF2/ASF (lanes 2 to 4) or ORF57 (lanes 5 to 7). (B) S100-complementation splicing assays of human β-globin pre-mRNA with 2, 8, or 15 pmol of either SF2/ASF (lanes 3 to 5), ORF57 (lanes 6 to 8), or a mixture (8 pmol each) of both (lane 9). The mobilities of the pre-mRNA and splicing products are indicated between the two splicing gels.