TABLE 1.
Primer pairs for RT-PCR analyses of viral or cellular RNA transcripts
| Oligonucleotide | Positiona | Gene | Sequence (5′→3′) |
|---|---|---|---|
| oST1 | 75182 (s) | K8 | TAATACGACTCACTATAGGG/ACCACCAAGAGGACCACACATTTC |
| oST3 | 75838 (as) | K8 | GTACTCACCCC/CACACAAAGTCTGGCATGGTTCTCCC |
| oKY27 | 71575 (s) | ORF50 | ACTGTCCAGGCAGCCACA |
| oKY28 | 72699 (as) | ORF50 | AGGTCACTGGGATCGTAGATTG |
| oKY30 | 127846 (as) | LANA | AGAGCAGCAGCTTGGTCCG |
| oST14 | 127264 (s) | LANA | GTCCCGACCTCAGGCGCATTCC |
| oKY46 | 126877 (s) | LANA | CTGGAGACTGCGTGGGTGG |
| oKY47 | 9 (s) | U1 | CTGGCAGGGGAGATACCATG |
| oKY48 | 163 (as) | U1 | AGGGGAAAGCGCGAACGCA |
| oKY49 | 13 (s) | U2 | CCTTTTGGCTAAGATCAAGTGTAG |
| oKY50 | 183 (as) | U2 | CACCGTTCCTGGAGGTACTG |
| oZMZ326 | 1 (s) | U6 | CATATCTCGC/GTGCTCGCTTCGGCAGCACA |
| oST197 | 106 (as) | U6 | AAAATATGGAACGCTTCACGA |
| oZMZ269 | 225 (s) | GAPDH | GTCATCAATGGAAATCCCATCACC |
| oZMZ270 | 502 (as) | GAPDH | TGAGTCCTTCCACGATACCAAA |
| oZMZ237 | 106 (s) | E6 | GTTTCAGGACCCACAGGAGC |
| oZMZ222 | 562 (as) | E6 | GTACTCACCCC/TGATTACAGCTGGGTTTC |
| oZMZ318 | 1b | β-Globin | ATCTGACTCGAG/CTTACATTTGCTTCTGACACAAC |
| oZMZ106 | 359b | β-Globin | GGGTTGCCCATAACAGCATCAGG |
| oKY32 | 2809 (s) | Survivin | GCATGGGTGCCCCGACGTTG |
| oKY33 | 12060 (as) | Survivin | GCTCCGGCCAGAGGCCTCAA |
| oKY34 | 51 (s) | IL-6 | AGGAGCCCAGCTATGAACTC |
| oKY35 | 410 (as) | IL-6 | GTCGAGGATGTACCGAATTTG |
a
s, sense; as, antisense.
b
Based on Promega pSP64 HβΔ6.