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Effect of primary antibody and gold bead size on VZV gE as seen in backscatter images. (A) Imaging of human MAb V2 and ultrasmall gold beads, showing 10 viral particles with numerous gold beads. (B) Imaging of mouse MAb 3B3 with ultrasmall gold beads, showing 24 viral particles labeled by fewer gold beads than in panel A. (C) Imaging of mouse MAb 3B3 with 10-nm gold beads, showing 12 viral particles with fewer gold beads than in either panel A or B.
