FIG. 9.

Detection of VZV gE on viral particles by electron microscopy. Samples were immunolabeled with MAb 3B3 and silver-enhanced ultrasmall gold beads and visualized by SEM. Arrows indicate gold beads. Not all beads are indicated. (A) Twelve viral particles with four or five beads each. (B) Thirteen particles with four or five beads each; the large complete particle in the middle has fourteen beads. (C) Enlargement of panel A with beads on three particles. (D) Enlargement of the large particle in panel B to further delineate the gold beads labeling gE. The immunolabeled samples in this figure and subsequent figures were prepared for viewing using protocol 1 (see Materials and Methods).