TABLE 1.

Myosin variants that were transiently expressed in N. benthamiana leaves

Myosin variant	Coding region nucleotidesa	Amino acid residuesb	Molecular mass with HA tag (kDa)
Complete VIII-1	1-3453	1-1150	142
VIII-1 IQc to C terminus	2479-3453	827-1150	41
VIII-1 CCd to C terminus	2830-3453	944-1150	28
VIII-1 GTD	2983-3453	995-1150	21
VIII-1 motor domain	1-2478	1-826	96
VIII-1 motor domain to CC	1-2982	1-994	116
Complete VIII-2	1-3591	1-1196	140
VIII-2 IQ to C terminus	2659-3591	887-1196	39
VIII-2 CC to C terminus	2944-3591	982-1196	28
VIII-2 GTD	3100-3591	1034-1196	22
VIII-2 motor domain	1-2592	1-864	102
VIII-2 motor domain to CC	1-3099	1-1033	121
VIII-B IQ to C terminus	2491-3471	831-1156	40
VIII-B CC to C terminus	2830-3471	944-1156	28
VIII-B GTD	2971-3471	991-1156	22
XI-2 IQ to C terminus	2203-4539	735-1512	92
XI-F IQ to C terminus	2197-4710	733-1569	100
XI-K IQ to C terminus	2197-4590	733-1529	102

^aNucleotide numbers start at the first nucleotide of the translation initiation codon according to the nucleotide sequences deposited in GenBank.

^bExcluding the triple HA tag.

^cIQ, the IQ motif; all IQ motifs in corresponding myosins, starting with the most N-terminal motifs, were included (Fig. 1).

^dCC, the coiled-coil motif.