FIG. 6.

siRNA inhibition of HIV-1 transcription for cells transfected with proviral DNA vectors. Panel A provides a schematic representation of the general cloning strategy to replace the env gene in pNL4-3 with the env genes of v120-A and v126-D. The resulting plasmids were then transfected in 293T cells in the presence or absence of siRNAs. Amp, ampicillin resistance cassette; P_CMV, cytomegalovirus promoter; Zeo, Zeocin resistance cassette. (B) The relative virus production as measured by RT activity was monitored in supernatants at days 2 and 5 after transfection and siRNA treatment.