FIG. 6.

Effect of mutations of the C/EBP motifs in the ori-Lyt fragment (3F) and deletion of RRE from the ori-Lyt fragment (11F) on the recruiting of cellular proteins to ori-Lyt DNA. (A) Biotinylated DNA fragments were prepared by PCR, with pOri-A (wild type) DNA and an RRE deletion derivative, which was described in Wang et al. (37), as templates. (B) Similarly, biotinylated DNA fragments were prepared by PCR with the pOri-A (wild type) DNA template and pOri-M12 and pOri-M1256, mutations on certain C/EBP motifs, which was described in Wang et al. (37). TPA-induced BCBL-1 nuclear extract was incubated with the DNA fragments conjugated on magnetic beads, washed, and eluted with D500 elution buffer. Samples were assayed by Western blotting with antibodies as indicated. C, an irrelevant DNA was included as a control.