FIG. 2.

CVA24v virions efficiently infect and bind to human ocular epithelial cells. (A) Adherent cells (2 × 10⁵) were incubated with 5,000 CVA24v virions per cell at 4°C for one hour, washed to remove unbound virions, and incubated for 16 to 18 h at 37°C. The cells were fixed with methanol, stained as described in Materials and Methods, and quantified in a fluorescence microscope. (B) Binding experiments were carried out exactly as described for Fig. 1A, but without neuraminidase and with different cells. The results are presented as means ± standard deviations of duplicate samples from at least three independent experiments. CPM, counts per minute.