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. 2008 Jan 9;82(6):3061–3068. doi: 10.1128/JVI.02470-07

FIG. 5.

FIG. 5.

Roles of SAα2,3Gal and SAα2,6Gal in binding of CVA24v to human ocular cells. (A) Flow cytometric analysis of expression of SAα2,3Gal (MAA II binding) and SAα2,6Gal (SNA binding) on NHC and HCE cells. Cells (5 × 105) in suspension were first incubated with biotinylated lectins, followed by streptavidin-FITC, prior to flow cytometric analysis. Data represent the geometric means ± standard deviations of the fluorescence measured in three experiments in duplicate. (B) Effect of SAα2,3Gal-binding MAA II and SAα2,3Gal-binding SNA on CVA24v virion binding to NHC and HCE cells. Cells (2 × 105) in suspension were preincubated with lectins prior to incubation with 5,000 35S-labeled CVA24v virions/cell. Cell-associated radioactivity (CPM [counts per minute] bound) was determined by using a scintillation counter. The results are presented as means ± standard deviations of duplicate samples from at least three independent experiments.