Figure 6.

Sensitivity of gebp, gpl1, and gpl2 mutants to cytokinin. A, In vitro growth of wild types and single, double, and triple mutants on MS in the absence (−K) or presence (+K) of kinetin (10 μg mL⁻¹). The triple mutant was distinguishable from wild type on lower concentrations (2–5 μg mL⁻¹) of kinetin and was observed on trans-zeatin (1 μg mL⁻¹) as previously described (Higuchi et al., 2004; To et al., 2004; Hutchison et al., 2006). Scale bars, 1 cm. B, Chlorophyll content of wild types and single, double, and triple mutants grown in the absence (white bars) or presence (black bars) of kinetin. Asterisks represent significant changes to corresponding wild-type controls in the presence or absence of kinetin using the ANOVA test. Plants were grown for 20 d. The value for wild-type Ler was set at 100%. The experiment was done at least three times with consistent results. [See online article for color version of this figure.]