Figure 4.

Wound-induced expression of JA-responsive genes in the jar1-1 mutant. Five-week-old wild-type, coi1-1, and jar1-1 plants were mechanically wounded as described in the legend to Figure 1B. Damaged leaves were collected for RNA extraction at the indicated times (h) after mechanical wounding. Blots were hybridized to probes for MYC2, JAZ5, JAZ7, two JA biosynthesis genes (AOS and OPR3), as well as ACT8 as a loading control. All blots except ACT8 were exposed to autoradiographic film for 6 h. The ACT8 blot was exposed for 16 h.