Figure 4.

Phenotypic analysis of vp8 seeds. A and B, Mature ears segregating vp8-R in SC (A) and W22 inbred (B) genetic backgrounds. C and D, Embryonic SAMs in developing wild-type and vp8-R (SC) embryos at 16 DAP. Scale bars = 200 μm. E and F, Abgerminal aleurone of wild-type and vp8-R mutant endosperms. G, RT-PCR analysis of meristem-related genes in vp8-R (SC) and vp8-R (W22) embryos. The RT-PCR condition for each gene was identical to that in Figure 3. The plants were grown simultaneously in a greenhouse.