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ABA content and expression of Vp14 and ZmABA8′oxA1a in vp8 embryos. A, ABA content of developing wild-type (W) and vp8 mutant embryos (M) at 16 DAP. Error bars indicate the mean deviation of replicate experiments. B, RT-PCR analysis of Vp14 and ZmABA8′ox A1a expression in vp8 embryos in the W22 and SC genetic backgrounds. Total RNA was prepared from embryos of field-grown vp8-umu1 developing seeds and greenhouse-grown vp8-R (W22) and vp8-R (SC) seeds.
