Figure 4.

Expression pattern of PIP2;1, PIP2;2, and PIP2;3 transcripts in P. patens wild type. For each gene, amplification with 20-mer specific primers on genomic DNA was included as a control (A, lane G). RT-PCR analyses were performed on RNA from 4-d-old protonema (A, lane p) or 3- to 4-week-old gametophores (A, lane g). The integrity of the total RNA in the two preparations was assumed by checking the presence of constitutively expressed 18S RNA (B, lanes p and g). All experiments were repeated three times with independent plant samples.