Skip to main content
. 2008 Mar;146(3):1207–1218. doi: 10.1104/pp.107.111351

Figure 5.

Figure 5.

Schematic representation of the PIP2;1, PIP2;2, and PIP2;3 genes, corresponding knockout constructs, and molecular analysis of the knockout mutants. A, Gene structure and knockout construct of the PIP2;1 gene. B, Gene structure and knockout construct of the PIP2;2 gene. C, Gene structure and knockout construct of the PIP2;3 gene. Dotted lines and numbers of base pair indicate the regions of the genes used for insertion in pNeo. The position of the primers (little arrows) is detailed in “Materials and Methods”. D, Expression analysis of the wild-type and mutant plants. Total RNA from wild-type, pip2;1, pip2;2, and pip2;3 plants was reverse transcribed and used for PCR. The experiments were repeated three times with independent wild-type and mutant plant samples. The integrity of the total RNAs in the preparations was assumed by checking the presence of constitutively expressed 18S RNA.