Figure 2.

VIGS efficiency of Naggpps (A) and Nafpps (B). RT-PCR was run on cDNA from leaf samples of plants treated with pTV00, pTVFPPS, and pTVGGPPS. Transcript accumulation levels were determined relative to the N. attenuata actin gene and presented as the mean value (±se) of five biological replicates. ***, Significantly different from pTV00 at P < 0.0001. Insets: RNA was isolated and pooled from leaves of two biological replicates for each line pTV00, pTVFPPS, and pTVGGPPS, hybridized with radio-labeled probes for Naggpps (A) and Nafpps (B), washed and analyzed with a Fujifilm FLA-3000 Phosphoimager. Lower lanes: Ethidium bromide-stained agarose gel with rRNA as a control for equal RNA loading.