Table II.
Genotypes of F1 plants from various crosses
| Cross
|
Gene
|
F1 Genotypea
|
||
|---|---|---|---|---|
| ♀ × ♂ | cpHsc70-1 |  |
 |
 |
| cpHsc70-2 |  |
 |
 |
|
 |
Observed no. | 79 | 0b | – |
 |
Observed no. | 56 | 17c | – |
 |
Observed no. | – | 104 | 0 |
a
+, Wild-type copy of the gene; Δ, gene with the T-DNA insertion.
b
No plant with the genotype +/Δcphsc70-1 +/Δcphsc70-2 was observed, indicating that no viable ovule with the genotype Δcphsc70-1 Δcphsc70-2 was produced.
c
The transmission efficiency of the double-knockout pollen was reduced compared to pollen with the Δcphsc70-2 single mutation.