Table II.
Cross
|
Gene
|
F1 Genotypea
|
||
---|---|---|---|---|
♀ × ♂ | cpHsc70-1 | |||
cpHsc70-2 | ||||
Observed no. | 79 | 0b | – | |
Observed no. | 56 | 17c | – | |
Observed no. | – | 104 | 0 |
+, Wild-type copy of the gene; Δ, gene with the T-DNA insertion.
No plant with the genotype +/Δcphsc70-1 +/Δcphsc70-2 was observed, indicating that no viable ovule with the genotype Δcphsc70-1 Δcphsc70-2 was produced.
The transmission efficiency of the double-knockout pollen was reduced compared to pollen with the Δcphsc70-2 single mutation.